In-Vitro Cytotoxic and Proliferative Activity of Three Plant Extract on Human Peripheral Blood Mononuclear Cells (PBMCs)

Plant products provide a vast source of therapeutics, but not without toxicity. This study evaluated the in vitro cytotoxic and proliferative activity selected plant extracts on human peripheral blood mononuclear cells (PBMCs). The PBMCs from healthy donors were exposed to varying concentrations (25 µg/ml, 50 100 µg/ml 200 µg/ml) aqueous young leaves Mangifera indica (MI) stem barks Commiphora kerstingii (CK), Lannea acida Acacia sieberiana formulation (LAASF). Trypan blue assay was used determine viability after isolation. Cytotoxicity proliferation determined using WST-8 assay. total viable cell count isolated this 8000 x 104 cells/ml while 96.15%. extract LAASF showed lowest percentage cytotoxicity (2.63%) at 25 concentration, followed by CK (2.70%), then MI (7.71%). There significant decrease mean absorbance scores across different (p=0.008), (p<0.0001) (p=0.01). statistically for (p=0.003) (p=0.005) compared control. However, no difference observed proliferation. Mean significantly decreased with an increase concentration extracts. have potential higher concentrations. exhibited than LAASF. An in-depth identify specific immune proliferated will improve credence study’s findings. Keywords: Herbal medicine, cytotoxicity, proliferation, humans, leukocytes